भाकृअनुप- भारतीय दलहन अनुसंधान संस्थान ICAR-Indian Institute of Pulses Research Pulses For Nutritional Security
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Dr. Vaibhav Kumar

Scientist

Area of specialization

Plant Biochemistry

Research area

Abiotic stress tolerance in pulses.

Significant research achievements

  • Identified key biochemical responses to heat stress in chickpea, demonstrating changes in glutamine synthetase (GS), glutamine oxoglutarate aminotransferase (GOGAT) and sucrose synthase (SS) activity associated with nitrogen assimilation and starch biosynthesis in developing seeds.
  • Established biochemical markers of heat tolerance in chickpea — total phenolics, flavonoids, antioxidant potential and phytosterols under heat stress.
  • Characterized heat-induced phenolic metabolites in chickpea using HPLC-DAD, demonstrating accumulation of syringic acid, sinapic acid, myricetin and other phenolics in heat-tolerant genotypes.
  • Identified molecular determinants of heat tolerance using transcriptomics, detecting genotype-specific differentially expressed genes including LOC101514603 and LOC101515262 (18.5 kDa HSPs).
  • Identified candidate genes associated with heat tolerance through genotype-specific expression of key genes in phenolic and phytosterol biosynthesis, providing potential molecular markers for heat-tolerant chickpea breeding.

Awards and recognitions

  • 2nd position, International Conference “Pulses as the Climate Smart Crop: Challenges and Opportunities”, ISPRD, Bhopal, 10–12 February 2020.
  • 3rd position, same conference, for biochemical estimation of fractionated milling by-product of pigeonpea.
  • CSIR-UGC JRF 2013 in Life Sciences; ICAR SRF 2013 and ICAR NET 2014 in Plant Biochemistry.

Profile link

Google Scholar

Personal information

Date of joining ICAR

01st January, 2016

Discipline and specialization

Plant Biochemistry

Training & Exposure

Chromatographic study of phytochemical in pulses, Enzyme assay, Enzyme Kinetics, Protein profiling, Spectrophotometric estimation of phytochemicals present in pulses.

Professional Information

Contribution to the scientific advancement

  • Standardized the biochemical parameters associated with heat stress in chickpea.
  • Involved in the estimation of bioactive components associated with the health-promoting properties and flatulence in field pea and common bean.
  • Biochemical estimation of pulse milling by-product for edible use.

Current area of research

Abiotic stress tolerance in pulses.

Publications

  • Kumar V., Ali K., Kumar A., Tewari K., Garg N.K., Changan S.S. and Tyagi A.(2018). Cloning and heterologous expression of Os-AP2/ERF-N22 drought inducible rice transcription factor in E. coli. Indian Journal of Agricultural Sciences 88 (10): 1515–20.
  • Tewari K., Kumar V., Kumar A. et al.(2018). Molecular cloning and functional analysis of the promoter of γ-Tocopherol Methyl Transferase (γ-TMT) gene of soybean (Glycine max), 3 Biotech, 8:325.
  • Tewari K., Dahuja A., Sachdev A., Kumar V., Ali K., Kumar A., and Kumari S.(2017). Molecular cloning, heterologous expression and functional characterization of gamma tocopherol methyl transferase (γ-TMT) from Glycine max. Protein Expression and Purification 140: 81-89.
  • Changan S.S., Ali K., Kumar V. et al. (2018). Abscisic acid biosynthesis under water stress: anomalous behavior of the 9-cis-epoxycarotenoid dioxygenase1 (NCED1) gene in rice. Biol Plant 62, 663–670.
  • Lamichaney A., Kumar V.., Katiyar P.K.(2018). Effect of seed priming induced metabolic changes on germination and field emergence of chickpea. Journal of Environmental Biology 39: 522–528.

Awards and Fellowships

  • Secured 2nd position in the International conference “Pulses as the climate smart crop: Challenges and Opportunities” organized by ISPRD held at Bhopal during Feb, 10-12, 2020. Tewari K., Kumar V., Charu S., and Parihar A. K. (2020). Profiling of fieldpea and cowpea genotypes for their bioactive contituents.
  • Secured 3rd position in the International conference “Pulses as the climate smart crop: Challenges and Opportunities” organized by ISPRD held at Bhopal during Feb, 10-12, 2020. Verma P., Kumar V., Parashar M., and Deepshikha.(2020). Biochemical estimation of fractionated milling by-product of Pigeon pea (Cajanuscajan L.).
  • CSIR-UGC JRF in 2013 in Life Sciences by UGC, New Delhi.
  • ICAR SRF in 2013 in Plant Biochemistry by ICAR, New Delhi.
  • ICAR NET in 2014 in Plant Biochemistry by ICAR, New Delhi.